Post-doctoral Training Fellow - Biochemistry/Biophysics and Cryo-Electron Microscopy

11/11/2018, 23:55

London
Structural Biology
Structural Biology of Cell Signalling
Full time
Fixed Term
12 Months
35
£30,715 - £39,082
The Institute of Cancer Research, London, is one of the world’s most influential cancer research institutes, with an outstanding record of achievement dating back more than 100 years. We provided the first convincing evidence that DNA damage is the basic cause of cancer, laying the foundation for the now universally accepted idea that cancer is a genetic disease. Today, The Institute of Cancer Research (ICR) leads the world at isolating cancer-related genes and discovering new targeted drugs for personalised cancer treatment.

Under the leadership of our Chief Executive, Professor Paul Workman FRS, the ICR is ranked as the UK’s leading academic research centre. Together with our partner The Royal Marsden, we are rated in the top four cancer centres globally.

The ICR is committed to attracting, developing and retaining the best minds in the world to join us in our mission – to make the discoveries that defeat cancer.

We seek to appoint a Postdoctoral Training Fellow to the research team led by Dr Sebastian Guettler in the Divisions of Structural and Cancer Biology. Work in the Guettler laboratory centres on the elucidation of signal transduction mechanisms relevant to the function of cancer cells, in particular the Wnt/b-catenin network and telomere homeostasis and their control by poly (ADP-ribosyl)ation. We take a multi-disciplinary approach that combines structural biology, both by X-ray crystallography and cryo-electron microscopy, biochemistry, biophysics and functional studies in mammalian cells.

 

You will undertake biochemical/biophysical and structural studies to investigate the molecular mechanisms of poly(ADP-ribose)polymerase (PARP) enzymes. A large part of the research project will be centred on the the structural and functional analysis of proteins/protein complexes by cryo-electron microscopy.

 

You should possess a PhD in a biological science such as biochemistry or molecular biology and have experience in structural biology, ideally single-particle electron microscopy. In addition, experience with recombinant DNA techniques, high-quality protein expression and purification, protein characterisation, such as protein-protein interaction and enzyme activity assays, is essential.

 

Appointment will be on a Fixed Term Contract for 12 months, with a possibility of an extension for a full three-year period, subject to available funding. The full salary scale is in the range from £30,715 - £39,082 p.a. inclusive. Starting salary will be based on previous postdoctoral experience. 


Research in the Divisions of Structural Biology and Cancer Biology focuses on the functional, biochemical, and structural characterisation of cellular processes relevant to cancer and cancer therapy. The Division of Structural Biology has managed facilities for protein production, with expertise in multi-subunit expression (insect cell, yeast and bacterial expression, including a 60-litre fermentor), protein crystallography (crystallisation robots, Bruker Microstar and CCD detector) and cryo-electron microscopy (FEI Tecnai F20 and T12). Regular access to shared cryo-electron microscopy facilities, such as eBIC, is available. Moreover, the Institute of Cancer Research is part of the London Consortium for Cryo-Electron Microscopy, hosting an FEI Titan Krios cryo-electron microscope equipped with a Volta phase plate, a Falcon III detector and a Gatan Bioquantum energy filter and K3 detector (available in early 2019). The Division is also well-equipped with instrumentation for biophysical analysis (e.g. ITC, fluorescence, multi-angle light scattering).

The Division of Cancer Biology provides a state-of-the-art infrastructure for mammalian cell culture, light microscopy, mammalian genetics, chemical biology and proteomics.

Informal enquiries can be made to sebastian.guettler@icr.ac.uk. Please do not submit your formal application by email,  applications must be submitted on the ICR Careers portal. Please  complete the  application form, giving specific examples of how you meet the person specification and summarising your research interests and motivation for applying. Please attach CV to your application form.

We consider all applications on merit and have a strong commitment to enhancing the diversity of our staff.
Additional Documentation for Candidates

This Program is closed to applications.